CD GENOMIC Reaches a New High in Simplifying Genome Sequencing Technique: 2b-RAD


CD Genomic Company is able to provide the 2b-RAD technique, the upgraded RAD technique to simplify genome sequencing technique thereby achieving genome-wide high-throughput SNP genotyping.

The use of simplified genome sequencing technology for large-scale high-throughput SNP genotyping is currently a hotspot in the field of animal and plant genomics research. Simplified genome sequencing refers to the fragmentation of genomic DNA by restriction enzyme digestion, selection of some of the characteristic fragments, and the development and classification of high-throughput genetic markers within the genome range by second-generation sequencing technology.

According to Mike Jorge, a core researcher of 2b-RAD in CD Genomics, “The widely used technology is the RAD technology invented by the University of Oregon. However, RAD technology has two main technical defects. One is the Cumbersome database construction process, which involves multi-step DNA separation and purification process, which will have serious impact on technical repeatability and reliability; Besides, what RAD technology creates is a restricted DNA fragment of different lengths, and since PCR technology itself has the preference of amplification template length (that is, preferential amplification of small fragment DNA), it will seriously affect the expansion after the PCR and sequencing phase of emulsion PCR, increasing the genome representation of DNA fragments.

Considering the above drawbacks, CD Genomics comes up with 2b-RAD technology. The 2b-RAD technology effectively overcomes the inherent flaws of the above RAD technology. The 2b-RAD technology utilizes type IIB restriction enzymes to generate an isometric 33-36 bp restriction cleavage by genomic digestion. These tags are enriched for downstream high-throughput sequencing reactions and analyzed by bioinformatics, hence achieving genome-wide high-throughput SNP genotyping.”

Mike also shared with us the technical flow of 2b-RAD during our interview. He said it can be generally divided into four steps including Enzymatic cleavage of genomic DNA using type II restriction enzymes; Recover specific length (33-35 bp) fragments; connection connector; PCR amplification using sample-specific primers and sequencing of amplified products.

“Compared with RAD technique, the 2b-RAD has wined more popularity among clients because of its advantages”, said the director of sale in CD Genomics, Judy Clark, “our 2b-RAN is complete library sequencing, no loss of restriction loci, with ultra-high-density coverage of the genome and more complete information. In addition, the process is simple and the technical repeatability is good: the result of one sample is highly repeatable. Another merit means its uniform tag sequencing depth, which ensures the reliability and accuracy of each tag.”

Now, the 2b-RAD technique from CD Genomics has already served several institutions. Mike also expressed they would continue to improve this technique and where there is a need for clients, there would be qualified service from CD.

About CD Genomics

CD Genomics is the Genomics services company specializing in the services of DNA sequencing, genotyping and DNA library construction. With both traditional and modern genotyping platforms like Illumina BeadStation 500, Beadxpress and Sequenom, CD Genomics can provide services in each and every area of the genotyping field with a focus on pharmacogenomics.

评论

发表评论

此博客中的热门博文

Separation of Nucleic Acids

图片
Abstract : The separation and purification of nucleic acids is a basic technology in biochemistry and molecular biology. As molecular biology techniques are widely used in biology, medicine, and related fields, nucleic acid separation and purification technologies have been further developed. The emergence of various new methods and improved traditional classical methods and commercial reagent methods has greatly promoted the development of molecular biology. Traditional nucleic acid separation requires ethanol precipitation, phenol/chloroform extraction, centrifugation, and column chromatography. The operation is cumbersome, time-consuming and labor-intensive, and it is difficult to achieve automatic separation and purification. Glass powder or glass beads have proven to be an effective nucleic acid adsorbent. In high salt solutions, nucleic acids can be adsorbed onto a glass substrate to facilitate separation of nucleic acids. In addition, surface functionalized polymeric magnetic ...
阅读全文

What is an anti-idiotypic antibody?

图片
With the development of drug research and development, there are more and more types of antibodies, such as camel antibodies , llama antibodies , monoclonal antibodies , recombinant antibodies , anti idiotype , anti-idiotypic antibodies   and the like. Definition of anti-idiotypic antibodies: The first is a specific antibody induced by the idiotype of the variable region of the antibody as an antigenic epitope. The second is specific antibodies that are unique to the unique type. An anti-idiotypic antibody is an antibody directed against a specific epitope group (referred to as an idiotype) on the V region of an antibody molecule. The "intra-image" relationship with the determinant molecules of the original antigen can mimic the structure and function of the antigen, and can be used as a novel vaccine. It is an antibody that directly recognizes and binds to the variable region of another antibody. It plays a pivotal role in drug discovery, mainly for: immunogenicity...
阅读全文

The Darling of the New Century: Nanoparticles

图片
  Do you understand these important concepts? Nanoparticles  refer to particles having a particle size between 1 and 100 nm, also known as ultrafine particles. They are in an over-extension between macroscopic objects and between micro-systems and macro-systems. They are a group of a small number of atoms or molecules, so they are neither a typical microscopic system nor an atypical macroscopic system. The basic meaning of nanoscience and technology  is to manipulate and arrange atomic and molecular innovations directly or indirectly in the nanometer category for making new substances. Nanomedicine  is an emerging edge interdisciplinary subject based on nanoscience or medicine. Its basic meaning refers to the use of nanotechnology theories and methods to carry out a new discipline of medical research and clinical treatment, which is mainly related to medicine in two aspects. Combination: First, the detection technology of disease; second, the treatment te...
阅读全文